Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology.
Identifieur interne : 002538 ( Main/Exploration ); précédent : 002537; suivant : 002539Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology.
Auteurs : Ruoqing Zhu [États-Unis] ; Olga Shevchenko ; Cathleen Ma ; Stéphane Maury ; Michael Freitag ; Steven H. StraussSource :
- Planta [ 1432-2048 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), Cytosine (métabolisme), Dormance des plantes (génétique), Feuilles de plante (anatomie et histologie), Gènes de plante (MeSH), Interférence par ARN (MeSH), Méthylation de l'ADN (génétique), Phénotype (MeSH), Populus (croissance et développement), Populus (génétique), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Suppression génétique (MeSH), Transformation génétique (MeSH), Transgènes (génétique), Végétaux génétiquement modifiés (MeSH).
- MESH :
- anatomie et histologie : Feuilles de plante.
- croissance et développement : Populus.
- génétique : ADN bactérien, Dormance des plantes, Méthylation de l'ADN, Populus, Protéines végétales, Transgènes.
- métabolisme : Cytosine, Protéines végétales.
- Gènes de plante, Interférence par ARN, Phénotype, Régulation de l'expression des gènes végétaux, Suppression génétique, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Cytosine (metabolism), DNA Methylation (genetics), DNA, Bacterial (genetics), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Phenotype (MeSH), Plant Dormancy (genetics), Plant Leaves (anatomy & histology), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (growth & development), RNA Interference (MeSH), Suppression, Genetic (MeSH), Transformation, Genetic (MeSH), Transgenes (genetics).
- MESH :
- chemical , genetics : DNA, Bacterial, Plant Proteins.
- chemical , metabolism : Cytosine, Plant Proteins.
- anatomy & histology : Plant Leaves.
- genetics : DNA Methylation, Plant Dormancy, Populus, Transgenes.
- growth & development : Populus.
- Gene Expression Regulation, Plant, Genes, Plant, Phenotype, Plants, Genetically Modified, RNA Interference, Suppression, Genetic, Transformation, Genetic.
Abstract
The Arabidopsis thaliana DDM1 (Decreased DNA Methylation) gene is necessary for the maintenance of DNA methylation and heterochromatin assembly. In Arabidopsis, ddm1 mutants exhibit strong but delayed morphological phenotypes. We used RNA interference (RNAi) to suppress transcripts of two orthologous DDM1 paralogs in Populus trichocarpa and examined effects on whole plant phenotypes during perennial growth and seasonal dormancy. The RNAi-PtDDM1 transgenic poplars showed a wide range of DDM1 transcript suppression; the most strongly suppressed line had 37.5 % of the expression of the non-transgenic control. Genomic cytosine methylation (mC %) was 11.1 % in the non-transgenic control, compared with 9.1 % for the transgenic event with lowest mC %, a reduction of 18.1 %. An evaluation of greenhouse growth directly after acclimation of in vitro grown plants showed no developmental or growth rate abnormalities associated with the decrease in PtDDM1 expression. However, after a dormancy cycle and growth outdoors, a mottled leaf phenotype appeared in some of the transgenic insertion events that had strongly reduced PtDDM1 expression and DNA methylation. The phenotypic consequences of reduced DDM1 activity and DNA methylation appears to increase with cumulative plant propagation and growth.
DOI: 10.1007/s00425-013-1858-4
PubMed: 23455459
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology.</title><author><name sortKey="Zhu, Ruoqing" sort="Zhu, Ruoqing" uniqKey="Zhu R" first="Ruoqing" last="Zhu">Ruoqing Zhu</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Shevchenko, Olga" sort="Shevchenko, Olga" uniqKey="Shevchenko O" first="Olga" last="Shevchenko">Olga Shevchenko</name></author><author><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name></author><author><name sortKey="Maury, Stephane" sort="Maury, Stephane" uniqKey="Maury S" first="Stéphane" last="Maury">Stéphane Maury</name></author><author><name sortKey="Freitag, Michael" sort="Freitag, Michael" uniqKey="Freitag M" first="Michael" last="Freitag">Michael Freitag</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23455459</idno><idno type="pmid">23455459</idno><idno type="doi">10.1007/s00425-013-1858-4</idno><idno type="wicri:Area/Main/Corpus">002678</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002678</idno><idno type="wicri:Area/Main/Curation">002678</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002678</idno><idno type="wicri:Area/Main/Exploration">002678</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology.</title><author><name sortKey="Zhu, Ruoqing" sort="Zhu, Ruoqing" uniqKey="Zhu R" first="Ruoqing" last="Zhu">Ruoqing Zhu</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Shevchenko, Olga" sort="Shevchenko, Olga" uniqKey="Shevchenko O" first="Olga" last="Shevchenko">Olga Shevchenko</name></author><author><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name></author><author><name sortKey="Maury, Stephane" sort="Maury, Stephane" uniqKey="Maury S" first="Stéphane" last="Maury">Stéphane Maury</name></author><author><name sortKey="Freitag, Michael" sort="Freitag, Michael" uniqKey="Freitag M" first="Michael" last="Freitag">Michael Freitag</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></analytic><series><title level="j">Planta</title><idno type="eISSN">1432-2048</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cytosine (metabolism)</term><term>DNA Methylation (genetics)</term><term>DNA, Bacterial (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Phenotype (MeSH)</term><term>Plant Dormancy (genetics)</term><term>Plant Leaves (anatomy & histology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>RNA Interference (MeSH)</term><term>Suppression, Genetic (MeSH)</term><term>Transformation, Genetic (MeSH)</term><term>Transgenes (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>Cytosine (métabolisme)</term><term>Dormance des plantes (génétique)</term><term>Feuilles de plante (anatomie et histologie)</term><term>Gènes de plante (MeSH)</term><term>Interférence par ARN (MeSH)</term><term>Méthylation de l'ADN (génétique)</term><term>Phénotype (MeSH)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Suppression génétique (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Transgènes (génétique)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytosine</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Feuilles de plante</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>DNA Methylation</term><term>Plant Dormancy</term><term>Populus</term><term>Transgenes</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Dormance des plantes</term><term>Méthylation de l'ADN</term><term>Populus</term><term>Protéines végétales</term><term>Transgènes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytosine</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Phenotype</term><term>Plants, Genetically Modified</term><term>RNA Interference</term><term>Suppression, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes de plante</term><term>Interférence par ARN</term><term>Phénotype</term><term>Régulation de l'expression des gènes végétaux</term><term>Suppression génétique</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Arabidopsis thaliana DDM1 (Decreased DNA Methylation) gene is necessary for the maintenance of DNA methylation and heterochromatin assembly. In Arabidopsis, ddm1 mutants exhibit strong but delayed morphological phenotypes. We used RNA interference (RNAi) to suppress transcripts of two orthologous DDM1 paralogs in Populus trichocarpa and examined effects on whole plant phenotypes during perennial growth and seasonal dormancy. The RNAi-PtDDM1 transgenic poplars showed a wide range of DDM1 transcript suppression; the most strongly suppressed line had 37.5 % of the expression of the non-transgenic control. Genomic cytosine methylation (mC %) was 11.1 % in the non-transgenic control, compared with 9.1 % for the transgenic event with lowest mC %, a reduction of 18.1 %. An evaluation of greenhouse growth directly after acclimation of in vitro grown plants showed no developmental or growth rate abnormalities associated with the decrease in PtDDM1 expression. However, after a dormancy cycle and growth outdoors, a mottled leaf phenotype appeared in some of the transgenic insertion events that had strongly reduced PtDDM1 expression and DNA methylation. The phenotypic consequences of reduced DDM1 activity and DNA methylation appears to increase with cumulative plant propagation and growth.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23455459</PMID><DateCompleted><Year>2014</Year><Month>01</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-2048</ISSN><JournalIssue CitedMedium="Internet"><Volume>237</Volume><Issue>6</Issue><PubDate><Year>2013</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology.</ArticleTitle><Pagination><MedlinePgn>1483-93</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00425-013-1858-4</ELocationID><Abstract><AbstractText>The Arabidopsis thaliana DDM1 (Decreased DNA Methylation) gene is necessary for the maintenance of DNA methylation and heterochromatin assembly. In Arabidopsis, ddm1 mutants exhibit strong but delayed morphological phenotypes. We used RNA interference (RNAi) to suppress transcripts of two orthologous DDM1 paralogs in Populus trichocarpa and examined effects on whole plant phenotypes during perennial growth and seasonal dormancy. The RNAi-PtDDM1 transgenic poplars showed a wide range of DDM1 transcript suppression; the most strongly suppressed line had 37.5 % of the expression of the non-transgenic control. Genomic cytosine methylation (mC %) was 11.1 % in the non-transgenic control, compared with 9.1 % for the transgenic event with lowest mC %, a reduction of 18.1 %. An evaluation of greenhouse growth directly after acclimation of in vitro grown plants showed no developmental or growth rate abnormalities associated with the decrease in PtDDM1 expression. However, after a dormancy cycle and growth outdoors, a mottled leaf phenotype appeared in some of the transgenic insertion events that had strongly reduced PtDDM1 expression and DNA methylation. The phenotypic consequences of reduced DDM1 activity and DNA methylation appears to increase with cumulative plant propagation and growth.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Ruoqing</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shevchenko</LastName><ForeName>Olga</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Cathleen</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Maury</LastName><ForeName>Stéphane</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Freitag</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven H</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>02</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C036483">T-DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>8J337D1HZY</RegistryNumber><NameOfSubstance UI="D003596">Cytosine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003596" MajorTopicYN="N">Cytosine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019175" MajorTopicYN="N">DNA Methylation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D057445" MajorTopicYN="N">Plant Dormancy</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="Y">anatomy & histology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="Y">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013489" MajorTopicYN="N">Suppression, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>01</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>02</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>1</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23455459</ArticleId><ArticleId IdType="doi">10.1007/s00425-013-1858-4</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nucleic Acids Res. 2008 Aug;36(14):4727-35</Citation><ArticleIdList><ArticleId IdType="pubmed">18632759</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 1999 May;22(1):94-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10319870</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2006 Jul;25(7):660-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16496153</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2010 Jul;5(7):806-16</Citation><ArticleIdList><ArticleId IdType="pubmed">20421728</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2000 Oct;6(4):791-802</Citation><ArticleIdList><ArticleId IdType="pubmed">11090618</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Genet Genomics. 2008 Jun;279(6):605-19</Citation><ArticleIdList><ArticleId IdType="pubmed">18357468</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2010 Feb;10(3):349-68</Citation><ArticleIdList><ArticleId IdType="pubmed">20148406</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2007 Aug 8;26(15):3641-52</Citation><ArticleIdList><ArticleId IdType="pubmed">17627280</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Dev. 1999 Nov 15;13(22):2971-82</Citation><ArticleIdList><ArticleId IdType="pubmed">10580004</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2001 May 10;411(6834):212-4</Citation><ArticleIdList><ArticleId IdType="pubmed">11346800</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2005 Jul;43(7):681-91</Citation><ArticleIdList><ArticleId IdType="pubmed">16046142</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2005 May;17(5):1549-58</Citation><ArticleIdList><ArticleId IdType="pubmed">15805479</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2012 Jan 17;13:27</Citation><ArticleIdList><ArticleId IdType="pubmed">22251412</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2011 May 18;30(10):1928-38</Citation><ArticleIdList><ArticleId IdType="pubmed">21487388</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Dev. 1998 Jun 1;12(11):1714-25</Citation><ArticleIdList><ArticleId IdType="pubmed">9620857</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Bot. 2002 Sep;90(3):417-21</Citation><ArticleIdList><ArticleId IdType="pubmed">12234154</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2006 Sep 22;126(6):1189-201</Citation><ArticleIdList><ArticleId IdType="pubmed">16949657</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):E981-8</Citation><ArticleIdList><ArticleId IdType="pubmed">22460791</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2011 Aug;1809(8):369-78</Citation><ArticleIdList><ArticleId IdType="pubmed">21414429</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2003 Jul;132(3):1260-71</Citation><ArticleIdList><ArticleId IdType="pubmed">12857808</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2002 Jan;12(1):122-31</Citation><ArticleIdList><ArticleId IdType="pubmed">11779837</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Mar 20;323(5921):1600-4</Citation><ArticleIdList><ArticleId IdType="pubmed">19179494</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2010 Oct 29;330(6004):622-7</Citation><ArticleIdList><ArticleId IdType="pubmed">21030646</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12406-11</Citation><ArticleIdList><ArticleId IdType="pubmed">8901594</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2004 Apr;24(4):401-7</Citation><ArticleIdList><ArticleId IdType="pubmed">14757579</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2003 Jan 10;278(2):823-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12403775</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Sep;136(1):2579-86</Citation><ArticleIdList><ArticleId IdType="pubmed">15375206</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2002 Aug;22(11):813-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12184986</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2009 Jun 16;4(6):e5932</Citation><ArticleIdList><ArticleId IdType="pubmed">19529764</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1990 Oct 5;215(3):403-10</Citation><ArticleIdList><ArticleId IdType="pubmed">2231712</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2010 Oct 28;6(10):e1001175</Citation><ArticleIdList><ArticleId IdType="pubmed">21060865</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2001 Dec;11(12):2041-9</Citation><ArticleIdList><ArticleId IdType="pubmed">11731494</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2004 Jul 22;430(6998):471-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15269773</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation><ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2006 Oct;142(2):788-96</Citation><ArticleIdList><ArticleId IdType="pubmed">16891552</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2003 Jun;8(6):263-71</Citation><ArticleIdList><ArticleId IdType="pubmed">12818660</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1993 Jun 25;260(5116):1926-8</Citation><ArticleIdList><ArticleId IdType="pubmed">8316832</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2007 Jan;39(1):61-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17128275</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2009 Apr;12(2):133-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19179104</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 1998 Jun;149(2):703-15</Citation><ArticleIdList><ArticleId IdType="pubmed">9611185</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2011 Feb;9(2):162-78</Citation><ArticleIdList><ArticleId IdType="pubmed">20573046</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecol Evol. 2013 Feb;3(2):399-415</Citation><ArticleIdList><ArticleId IdType="pubmed">23467802</ArticleId></ArticleIdList></Reference><Reference><Citation>J Comput Biol. 2005 Oct;12(8):1047-64</Citation><ArticleIdList><ArticleId IdType="pubmed">16241897</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Dev. 2001 Mar 1;15(5):591-602</Citation><ArticleIdList><ArticleId IdType="pubmed">11238379</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2008 Mar;66(5):463-73</Citation><ArticleIdList><ArticleId IdType="pubmed">18236011</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2000 Mar;12(3):357-69</Citation><ArticleIdList><ArticleId IdType="pubmed">10715322</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2003 May;34(3):383-92</Citation><ArticleIdList><ArticleId IdType="pubmed">12713544</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 1999 Feb;151(2):831-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9927473</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2004 Aug 18;4:14</Citation><ArticleIdList><ArticleId IdType="pubmed">15317655</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><noCountry><name sortKey="Freitag, Michael" sort="Freitag, Michael" uniqKey="Freitag M" first="Michael" last="Freitag">Michael Freitag</name><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name><name sortKey="Maury, Stephane" sort="Maury, Stephane" uniqKey="Maury S" first="Stéphane" last="Maury">Stéphane Maury</name><name sortKey="Shevchenko, Olga" sort="Shevchenko, Olga" uniqKey="Shevchenko O" first="Olga" last="Shevchenko">Olga Shevchenko</name><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></noCountry><country name="États-Unis"><region name="Oregon"><name sortKey="Zhu, Ruoqing" sort="Zhu, Ruoqing" uniqKey="Zhu R" first="Ruoqing" last="Zhu">Ruoqing Zhu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002538 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002538 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23455459
|texte= Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23455459" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |